Detailed view for Tb927.9.4620

Basic information

TDR Targets ID: 15612
Trypanosoma brucei, NEDD8 activating enzyme subunit, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.9876 | Length (AA): 467 | MW (Da): 51296 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00899   ThiF family

Gene Ontology

Mouse over links to read term descriptions.
GO:0019781   NEDD8 activating enzyme activity  
GO:0004839   ubiquitin activating enzyme activity  
GO:0005739   mitochondrion  
GO:0016881   acid-amino acid ligase activity  
GO:0008641   small protein activating enzyme activity  
GO:0005524   ATP binding  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  
GO:0006464   protein modification process  
GO:0045116   protein neddylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128095)

Species Accession Gene Product
Arabidopsis thaliana AT5G19180   NEDD8-activating enzyme E1 catalytic subunit
Babesia bovis BBOV_I001030   ThiF family domain containing protein
Brugia malayi Bm1_35445   Ectopic membrane ruffles in embryo protein 1
Candida albicans CaO19.11685   Ubiquitin activating enzyme
Candida albicans CaO19.4209   Ubiquitin activating enzyme
Caenorhabditis elegans CELE_F11H8.1   Protein RFL-1
Cryptosporidium hominis Chro.80201   ubiquitin-activating enzyme
Cryptosporidium parvum cgd8_1730   Uba3p like ubiquitin activating enzyme E1
Dictyostelium discoideum DDB_G0283891   NEDD8-activating enzyme E1 catalytic subunit
Drosophila melanogaster Dmel_CG13343   Ubiquitin activating enzyme 3
Echinococcus granulosus EgrG_000970100   NEDD8 activating enzyme E1 catalytic subunit
Entamoeba histolytica EHI_098550   ubiquitin-activating enzyme, putative
Echinococcus multilocularis EmuJ_000970100   NEDD8 activating enzyme E1 catalytic subunit
Homo sapiens ENSG00000144744   ubiquitin-like modifier activating enzyme 3
Leishmania braziliensis LbrM.01.0690   ubiquitin activating enzyme, putative
Leishmania donovani LdBPK_010730.1   ubiquitin activating enzyme, putative
Leishmania infantum LinJ.01.0730   ubiquitin activating enzyme, putative
Leishmania major LmjF.01.0710   ubiquitin activating enzyme, putative
Leishmania mexicana LmxM.01.0710   ubiquitin activating enzyme, putative
Loa Loa (eye worm) LOAG_01766   ectopic membrane ruffles in embryo protein 1
Mus musculus ENSMUSG00000030061   ubiquitin-like modifier activating enzyme 3
Neospora caninum NCLIV_040320   ubiquitin-activating enzyme, putative
Oryza sativa 4325169   Os01g0271500
Plasmodium berghei PBANKA_0713800   NEDD8-activating enzyme E1 catalytic subunit, putative
Plasmodium falciparum PF3D7_0817000   NEDD8-activating enzyme E1 catalytic subunit, putative
Plasmodium knowlesi PKNH_0503400   NEDD8-activating enzyme E1 catalytic subunit, putative
Plasmodium vivax PVX_089560   ubiquitin-activating enzyme E1C, putative
Saccharomyces cerevisiae YPR066W   NEDD8-activating protein UBA3
Schistosoma japonicum Sjp_0033200   ko:K10686 ubiquitin-activating enzyme E1 C, putative
Schistosoma japonicum Sjp_0090040   NEDD8-activating enzyme E1 catalytic subunit, putative
Schistosoma japonicum Sjp_0124080   ko:K06917 tRNA 2-selenouridine synthase, putative
Schistosoma japonicum Sjp_0131750   NEDD8-activating enzyme E1 catalytic subunit, putative
Schistosoma mansoni Smp_151760   ubiquitin-activating enzyme E1C
Schmidtea mediterranea mk4.001703.08   NEDD8-activating enzyme E1 catalytic subunit
Trypanosoma brucei gambiense Tbg972.9.2440   ubiquitin-activating enzyme e1, putative
Trypanosoma brucei Tb927.9.4620   NEDD8 activating enzyme subunit, putative
Trypanosoma congolense TcIL3000_9_1520   ubiquitin-activating enzyme E1, putative
Trypanosoma cruzi TcCLB.510155.120   ubiquitin activating enzyme, putative
Trypanosoma cruzi TcCLB.511585.130   ubiquitin activating enzyme, putative
Toxoplasma gondii TGME49_264880   NEDD8-activating enzyme E1 catalytic subunit
Theileria parva TP01_0601   hypothetical protein, conserved
Trichomonas vaginalis TVAG_161040   molybdopterin biosynthesis moeb protein, putative

Essentiality

Tb927.9.4620 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.160.3210 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.160.3210 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.160.3210 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb09.160.3210 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F11H8.1 Caenorhabditis elegans embryonic lethal wormbase
TGME49_264880 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens ubiquitin-like modifier activating enzyme 3 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0638 0.2833 1
0.0484 0.302 1
0.0576 0.2831 1
0.0488 0.3084 1
0.0283 0.2774 1
0.0089 0.2921 1
0.018 0.2909 1
0.0576 0.2831 1
0.0576 0.2831 1
0.0434 0.2876 1
0.0582 0.3094 1
0.0393 0.2696 1
0.0471 0.306 1
0.0635 0.2962 1
0.0414 0.2967 0.6786
0.0432 0.2622 1
0.0245 0.2504 0.3793
0.0635 0.2962 1
0.0414 0.2967 0.6786
0.0619 0.2941 1
0.0165 0.2876 1
0.0373 0.3034 0.7236
0.0397 0.2671 1
0.0233 0.2768 1
0.0253 0.3022 0.6218
0.018 0.2909 1
0.0528 0.3068 1
0.0373 0.3034 0.7236
0.0193 0.2876 1
0.0471 0.306 1
0.046 0.2921 1
0.0121 0.2891 1
0.0625 0.2979 1
0.0246 0.2564 0.2764
0.0502 0.2876 1
0.0686 0.3086 1
0.0546 0.3094 1
0.0613 0.2543 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.9.4620 (Trypanosoma brucei), NEDD8 activating enzyme subunit, putative
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